产品说明书
FAQ
COA
已发表文献
Tumor necrosis factor alpha (TNF-α), also known as cachectin, is a cachectin, is a polypeptide cytokine produced by monocytes and macrophages. TNFα receptor signaling can simultaneously activate caspase 8, the transcription factor, NF-κB and the kinase, JNK. Human TNF-α shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. Inflammation is associated with insulin resistance, and TNF-α and IL-6 may affect glucose uptake. A wide variety of evidence has pointed to a critical role of TNF-α in tumour proliferation, migration, invasion and angiogenesis.
Product Properties
|
Synonyms |
TNFSF2, Cachectin, Differentiation-inducing Factor, DIF, Necrosin, Cytotoxin |
|
Accession |
P48094 |
|
GeneID |
715467 |
|
Source |
E.coli-derived Rhesus Macaque TNF-α/TNFSF2, Val77-Leu233. |
|
Molecular Weight |
Approximately 17.3 kDa. |
|
AA Sequence |
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D. Fully biologically active when compared to standard. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
HB220318
