Porcine HGF(NK1) Protein(Animal Free) 重组猪源HGF(NK1)蛋白（92322ES）

Hepatocyte growth factor (HGF) is a potent epithelial mitogen whose actions are mediated through its receptor, the proto-oncogene c-Met. Full-length HGF is a 90-kD protein composed of an NH2-terminal domain, four consecutive kringle domains, and a carboxy-terminal protease-like domain. NK1 was converted to an agonist by adding heparin to the culture medium. The interaction of NK1 and heparin was further studied in BaF3 cells, which express little or no cell surface heparan sulfate proteoglycans. In BaF3 cells transfected with a plasmid encoding human c-Met, heparin and NK1 synergized to stimulate DNA synthesis and cell proliferation.

高活性、高纯度、低内毒素、高批间一致性

                                     SDS-PAGE

2 μg/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.

                                       RP-HPLC

The purity of  Porcine  HGF(NK1) is greater than 95% as determined by RP-HPLC.

                                       Bioactivity

Measured by its ability to induce serum response element (SRE) reporter activity in transfected HEK293T human embryonic kidney cells. The EC50 for this effect is less than 30-37ng/ml.

-25 ~ -15℃保存，收到货之后有效期1年。复溶后， 2 ~ 8 °C保存，7 天有效期。复溶后， -85 ~ -65℃保存，3个月有效期。建议首次使用时将蛋白等分，避免重复冻融循环。