产品简介

 

Hepatocyte Growth Factor(HGF), also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1 - 5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain.

 

性能参数

 

Synonyms	F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A;
Uniprot No.	P08048
Source	Human HGF Protein is expressed from HEK293 without tag. It contains Gln33-Arg495 (alpha)&Vla496-Leu728(beta).
Molecular Weight	The protein has a predicted 79.3 kDa
Purity	> 95% as determined by SDS-PAGE.
Biological Activity	Measured in a cell proliferation assay using mIMCD-3 mouse epithelial cells. The EC50 for this effect is 1-10 ng/mL.
Endotoxin	< 0.01 EU per 1μg of the protein by the LAL method.
Formulation	Dissolved in sterile PBS buffer.
Concentration	0.2 mg/ml

 

储存条件

 

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

 

1. Please operate with lab coats and disposable gloves,for your safety.   
2. This product is for research use only.

Ver. CN20231214