The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck.

推荐稀释比 WB:1/500-1/1000;IHC:1/50-1/100;ICC-1/100-1/200

Western blot analysis of Phospho-ZAP70 (Tyr319) in HL-60 lysates using Phospho-ZAP70 (Tyr319) antibody.

Immunofluorescence analysis of Phospho-ZAP70 (Tyr319) in Hela using Zap70(Phospho-Tyr319) antibody,and DAPI (blue).

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.