Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes. Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore .The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3.May serve as an epigenetic mark that propagates centromere identity through replication and cell division.Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis.

推荐稀释比  WB：1/500-1/1000  IHC-P：1/50-1/100

Western

Western blot analysis of CENP-A in lysates of HeLa,A375,HCT-116,Mouse colon and Rat colon using CENP-A antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Mouse small intestine using CENP-A antibody.High pressure and temperature Tris/EDTA (PH 9.0) used for antigen retrieval.The brown color represents the positive signal observed with CENP-A antibody.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。