产品描述
载脂蛋白E(apoE)是外周和中枢神经系统中的脂质载体。载脂的apoE脂蛋白颗粒与多种细胞表面受体结合,以支持脑膜稳态和损伤修复。考虑到患病率和相对风险大小,APOE基因的α4等位基因是晚发型阿尔茨海默病(AD)最强的遗传风险因素。
性能参数
蛋白别名(Synonyms) |
APOE2; APOE; Apo-E |
种属(Species) |
Human |
表达系统(Source) |
HEK293 |
表达序列(Sequence) |
Lys19-His317(R176C) |
Accession |
P02649-1 |
标签(Tag) |
C-His |
分子量(Molecular Weight) |
The protein has a predicted MW of 35.28 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Bis-Tris PAGE result. |
内毒素(Endotoxin) |
< 1 EU/μg by the LAL method. |
纯度(Purity) |
> 95% as determined by Bis-Tris PAGE; |
活性(Activity) |
SPR Data: Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE2, His Tag with an affinity constant of 27.11 nM as determined in SPR assay. |
制剂(Formulation) |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
外观(Appearance) |
冻干粉 |
储存条件
-25~-15℃保存,收到货之后有效期1年。复溶后, 2 ~8 °C保存,7天有效期。复溶后, -85~-65℃保存,3个月有效期。
复溶方法
开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
注意事项
1.为了您的安全和健康,请穿实验服并戴一次性手套操作。
2.本产品仅作科研用途。
Ver. CN20240919