性能参数
蛋白别名(Synonyms) |
Apo-E;Apolipoprotein E; APOE |
表达系统及表达区间(Source) |
Human APOE4/Apolipoprotein E Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Lys19-His317.[Accession | AAB59397.1] |
分子量(Molecular Weight) |
The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Tris-Bis PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by Tris-Bis PAGE |
制剂(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS, 2mM DTT, 5mM CHAPS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
复溶方法(Reconstitution) |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3-6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
Ver. CN20231201