Cloning, analysis, and anti–duck Tembusu virus innate immune response of Cherry Valley duck tripartite motif-containing 32
Tianxu Li, Xiaofang Hu, Tingting Zhang, Xingdong Song, Huihui Zhang, Ning Dai, Wei Sheng, Xiaolan Hou, Liangmeng Wei
Journal：POULTRY SCIENCE
IF：3.35
DOI：10.1016/j.psj.2021.101048
PMID：33744610
Published：2021-02-15
research field：神经科学分子生物学生物物理学
Abstract
Tripartite motif-containing 32 ( TRIM32 ) is an E3 ubiquitin ligase with multiple functions. In this study, we amplified TRIM32 gene from the Cherry Valley duck, and its cDNA sequence contained an open reading frame of 1,950 bp that encodes 649 amino acids. Duck TRIM32 ( duTRIM32 ) mRNA was expressed in all tissues tested. A series of immune-related genes that were induced by viral infection, including interferon alfa, IL-1β, retinoic acid–inducible gene-I, Mx, and OAS, were regulated by duTRIM32 expression. DuTRIM32 overexpression inhibits duck Tembusu virus ( DTMUV ) replication in the early stages of viral infection. Knockdown of duTRIM32 expression by siRNA reduced the ability of duck embryo fibroblast cells to mount a type Ⅰ interferon response to DTMUV. Therefore, our results suggest that the duTRIM32 -mediated signal pathway plays an essential role in DTMUV infection-induced innate immune response.
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