Recombinant Mouse RANKL/TNFSF11/CD254 Protein,His Tag

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产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2

表达区间及表达系统(Source)

Mouse RANKL/TNFSF11/CD254 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Tyr70-Asp316.[Accession | AAC40113.1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 28.97 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Mouse RANKL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

外观(Appearance)

冻干粉

 

储存条件

-25~-15℃保存,收到货之后有效期1年。复溶后, -85~-65℃保存,3个月有效期。

复溶方法

开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。

注意事项

1.为了您的安全和健康,请穿实验服并戴一次性手套操作。

2.本产品仅作科研用途。    

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