性能参数
分子别名(Synonyms) |
ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2 |
表达区间及表达系统(Source) |
Mouse RANKL/TNFSF11/CD254 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Tyr70-Asp316.[Accession | AAC40113.1] |
分子量大小(Molecular Weight) |
The protein has a predicted MW of 28.97 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on SDS-PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by SDS-PAGE and HPLC. |
活性(Activity) |
ELISA Data: Immobilized Mouse RANKL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. |
制剂(Formulation) |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
外观(Appearance) |
冻干粉 |
储存条件
-25~-15℃保存,收到货之后有效期1年。复溶后, -85~-65℃保存,3个月有效期。
复溶方法
开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
注意事项
1.为了您的安全和健康,请穿实验服并戴一次性手套操作。
2.本产品仅作科研用途。