Recombinant Hamster FGF21Protein,His Tag

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产品说明书

FAQ

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已发表文献

 

性能参数

分子别名(Synonyms)

UNQ3115; PRO10196; FGF-21; FGF21

表达区间及表达系统(Source)

Hamster FGF21 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Arg29-Ser209.[Accession | XP_005084765.1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 20.80 kDa. Due to glycosylation, the protein migrates to 21-30 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE,> 90% as determined by HPLC

活性(Activity)

SPR Data:Hamster FGF21, His Tag immobilized on CM5 Chip can bind Human Beta Klotho, His Tag with an affinity constant of 264.50 nM as determined in SPR assay .

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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