产品说明书
FAQ
COA
已发表文献
性能参数
|
表达区间及表达系统（Source） |
Biotinylated Human IL-12 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ile23-Ser328(IL-12B)/Arg23-Ser219(IL-12A).[Accession | P29460(IL-12B)&P29459(IL-12A)] |
|
分子量大小（Molecular Weight） |
The protein has a predicted MW of 34.7(IL-12B) kDa / 25.5 (IL-12A) kDa. Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result. |
|
内毒素（Endotoxin） |
Less than 1EU per μg by the LAL method. |
|
纯度（Purity） |
> 95% as determined by Tris-Bis PAGE and HPLC |
|
活性（Activity） |
ELISA Data: Immobilized Anti-IL-12 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC50 of 13.0ng/ml determined by ELISA. |
|
制剂（Formulation） |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
重构方法（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230625
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