产品说明书
FAQ
COA
已发表文献
性能参数
|
表达区间及表达系统（Source） |
Human CD47 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Gln19-Pro139.[Accession | Q08722-1] |
|
分子量大小（Molecular Weight） |
The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS- PAGE result. |
|
内毒素（Endotoxin） |
<1EU per μg by the LAL method. |
|
纯度（Purity） |
> 95% as determined by SDS- PAGE and HPLC |
|
活性（Activity） |
ELISA Data: Immobilized Human SIRP alpha V8, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 21.2ng/ml determined by ELISA. SPR Data: Human CD47, hFc Tag captured on Protein A chip via Anti-His Antibody can bind Human Sirpa V2, His Tag with an affinity constant of 13.8nM as determined in SPR assay |
|
制剂（Formulation） |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
重构方法（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230625
推荐商品
