产品说明书
FAQ
COA
已发表文献
性能参数
|
分子别名（Synonyms） |
CD279; PD1;PDCD1; PD-1 |
|
表达区间及表达系统（Source） |
Human PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Leu25-Gln167.[Accession | Q15116-1] |
|
分子量大小（Molecular Weight） |
The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on SDS-PAGE result. |
|
内毒素（Endotoxin） |
Less than 1EU per μg by the LAL method. |
|
纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC. |
|
活性（Activity） |
ELISA Data: Immobilized Human PD-1, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA .Immobilized Human PD-1, hFc Tag at 1μg/ml (100ul/Well). Dose response curve for Human PD-L1, mFc Tag with the EC50 of 0.1μg/ml determined by ELISA. |
|
制剂（Formulation） |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
重构方法（Reconstitution） |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
