脱脂奶粉（Skim Milk）是将鲜牛奶脱去脂肪再干燥而成，常用于微生物培养基的制备，以及用作免疫实验如Western Blot，ELISA的封闭剂。普通脱脂奶粉由于嗜热微生物的存在常引起有偏差的培养结果，因此不适用于细菌培养基。然而本品经特殊喷雾干燥法所得，不含任何嗜热微生物，达到细菌培养级。
脱脂奶粉可单独或者作为一种复杂细菌培养基的添加成分。基于凝固和酪蛋白水解的原理，脱脂奶粉可用于分化鉴定食品和乳制品微生物。常与pH，石蕊和溴甲酚紫混合使用来分析产酸情况，也常与亚甲蓝和刃天青混合使用建立氧化还原指示系统。也可以用作微生物菌种的保藏。
本品用于封闭的常用浓度为5%（v/v）。
| 外观（Appearance） | 乳白或白色粉末 |
| 水分（moisture） | ＜5% |
| 脂肪含量（Fat content） | ≤1.5% |
| 蛋白质（Protein） | ≥34% |
| 溶解性（Solubility） | 溶于水 |
室温运输。室温密封干燥保存。
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