产品说明书
FAQ
COA
已发表文献
NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain. NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset that account for more that 85% of NK cells found in peripheral blood and spleen. NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation. Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK-mediated lysis of immature dendritic cells.
产品性质
|
别名（Synonyms） |
CD337, LY117, NCR3, NKp30, 1C7, MALS |
|
Uniprot No. |
|
|
表达区间及表达系统（Source） |
Recombinant Human NKp30 / NCR3 / CD337 Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Leu19-Thr138. |
|
分子量(Molecular Weight) |
Approximately 39.8 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result. |
|
外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
|
活性（Activity） |
1. ELISA Data: Immobilized Human NKp30, hFc Tag at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Biotinylated Anti-NKp30 Antibody, hFc Tag with the EC50 of 13.4 ng/mL determined by ELISA. 2. SPR Data: Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Human B7-H6, His Tag with an affinity constant of 23.10 nM as determined in SPR assay (Biacore T200). |
|
内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
HB220707
