产品说明书
FAQ
COA
已发表文献
ApoE is a major protein component of serum LDL, VLDL, HDL, and chylomicrons. It is produced predominantly by hepatocytes, macrophages, and non-neuronal cells in the CNS. ApoE-containing particles transport triglycerides and cholesterol to peripheral tissues for cellular uptake and catabolism. Mature human ApoE is a 37 kDa glycoprotein. ApoE3 is the most abundant of the three common alleles in human; ApoE2 and ApoE4 differ by single aa substitutions. Mature human ApoE shares 71% aa sequence identity with mouse and rat ApoE. ApoE3 dampens the TNF-alpha induced inflammatory response in vascular endothelial cells.
产品性质
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别名（Synonyms） |
Apolipoprotein E, Apo-E, APOE, apolipo E |
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Uniprot No. |
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表达区间及表达系统（Source） |
Recombinant Human APOE / Apolipoprotein E Protein is expressed from HEK293 Cells with His tag at the N-terminal. It contains Lys19-His317. |
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分子量(Molecular Weight) |
Approximately 35.3 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. |
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外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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纯度（Purity） |
> 95% as determined by SDS-PAGE |
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活性（Activity） |
1. SPR Data: Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE, His Tag with an affinity constant of 1.004 μM as determined in SPR assay (Biacore T200). 2. SPR Data: Human LILRB4, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE, His Tag with an affinity constant of 115.9 nM as determined in SPR assay (Biacore T200). |
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内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
HB220707
