产品说明书
FAQ
COA
已发表文献
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects.
产品性质
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别名（Synonyms） |
FR-alpha, FR alpha, FR α, FOLR, FBP, Folbp1, KB cells FBP, MOv18, Fbp1, Folate receptor alpha |
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Uniprot No. |
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表达区间及表达系统（Source） |
Recombinant Human FOLR1 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Arg25-Met233. |
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分子量(Molecular Weight) |
Approximately 27.5 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result. |
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外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
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活性（Activity） |
ELISA Data: Immobilized Human FOLR1, His Tag at 0.5 μg/mL (100 μL/Well) on plate. Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 11.9 ng/mL determined by ELISA. |
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内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
Tris-Bis PAGE
HB220706
