产品说明书
FAQ
COA
已发表文献
Transforming Growth Factor-alpha (TGF-α) , also known as sarcoma growth factor, TGF-type I and ETGF, is a member of the EGF family of cytokines. TGF-α is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells.Mature TGF-alpha shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects.
Product Properties
|
Synonyms |
Transforming Growth Factor alpha; TGF-a; TGF-type I; ETGF |
|
Accession |
P01135 |
|
Gene ID |
7039 |
|
Source |
E.coli-derived Human TGF-a protein, Vla40-Ala89 |
|
Molecular Weight |
Approximately 5.8 kDa, on SDS-PAGE under reducing conditions. |
|
AA Sequence |
V VSHFNDCPDS HTQFCFHGTC RFLVQEDKPA CVCHSGYVGA RCEHADLLA |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
> 95% as determined by SDS-PAGE |
|
Biological Activity |
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is < 0.4 ng/mL. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized after extensive dialysis against PBS. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -80℃ for 1 year from date of receipt.
1week, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1.For your safety and health, please wear lab coats and disposable gloves for operation.
2.For research use only!
HB230921
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