Recombinant Human sCD23

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产品说明书

FAQ

COA

已发表文献

CD23 is a type 2 transmembrane C-type lectin that binds IgE, CD21, CD11b and CD11c. It is expressed on a variety of hematopoietic cells and functions in allergic immune responses. Recombinant human sCD23 is a 19.2 kDa non-glycosylated protein containing 172 amino-acid residues.

 

Product Properties

Synonyms

FCER2

Accession

P06734

GeneID

2208

Source

E.coli-derived human sCD23 protein, Met1-Ser321.

Molecular Weight

Approximately 19.2 kDa.

AA Sequence

MELQVSSGFV CNTCPEKWIN FQRKCYYFGK GTKQWVHARY ACDDMEGQLV SIHSPEEQDF LTKHASHTGS WIGLRNLDLK GEFIWVDGSH VDYSNWAPGE PTSRSQGEDC VMMRGSGRWN DAFCDRKLGA WVCDRLATCT PPASEGSAES MGPDSRPDPD GRLPTPSAPL HS

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

The ED50 determined by its ability to induce TNF-alpha production by human PBMCs. Fully biologically active when compared to standard.

Endotoxin

< 0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

 

HB220510

400-6111-883