性能参数
表达区间及表达系统(Source) |
Recombinant Human IL-2 R beta/CD122 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Ala27-Asp239.[Accession | NP_000869] |
分子量大小(Molecular Weight) |
The protein has a predicted MW of 51.35 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by Tris-Bis PAGE and HPLC |
活性(Activity) |
SPR Data: Human IL-2 R beta, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15, No Tag with an affinity constant of 2.88 nM as determined in SPR assay SPR Data: Human IL-2 R beta, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.216 μM as determined in SPR assay . |
制剂(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法(Reconstitution) |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230602