Recombinant Human GDF-2/BMP-9 Protein 重组人骨形成蛋白9

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产品描述

 

骨形态发生蛋白(BMP)在人类卵巢卵泡的不同细胞类型中表达,在卵巢功能的调节中起重要作用。BMP-9 又称生长分化因子-2(GDF-2),属于转化生长因子-β(TGF-β)超家族。BMP-9 主要在肝脏中合成并分泌到血液中,从而调节各种生理和病理功能。

 

性能参数

 

蛋白别名(Synonyms)

GDF-2; GDF2; BMP-9; BMP9

种属(Species)

Human

表达系统(Source)

HEK293

表达序列(Sequence)

Ser320-Arg429

Accession

Q9UK05

标签(Tag)

No Tag

分子量(Molecular Weight)

The protein has a predicted MW of 12.08 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.

内毒素(Endotoxin)

< 1 EU/μg by the LAL method.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

活性(Activity)

ELISA Data: Immobilized Human GDF-2, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 9.6ng/ml determined by ELISA.

Cell Based Assay: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.4-1.6 ng/mL .

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization.

外观(Appearance)

冻干粉

 

 

储存条件

 

-25~-15℃保存,收到货之后有效期1年。复溶后, 2 ~8 °C保存,7天有效期。复溶后, -85~-65℃保存,3个月有效期。

 

复溶方法

 

开盖之前,先离心。建议复溶浓度超过100μg/ml。将冻干的蛋白质溶解在4mM HCl中。

 

注意事项

 

1.为了您的安全和健康,请穿实验服并戴一次性手套操作。

2.本产品仅作科研用途。

 

 

Ver. CN20240919

400-6111-883