产品说明书
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产品描述
生长/分化因子 8 (GDF8)，又称肌生长抑制素，可负向调节肌肉质量。GDF8 通过与其 N 端前结构域相互作用而处于潜伏状态。与众多 TGF-β 家族成员一样，GDF8 是一种二硫键连接二聚体，合成为前体蛋白，需要通过弗林样蛋白酶裂解才能产生 N 端前结构域和 C 端成熟的信号传导结构域。
性能参数
|
蛋白别名（Synonyms） |
Myostatin; GDF8; Mstn; GDF-8; Myostatin |
|
种属（Species) |
Human/Mouse/Rat |
|
表达系统（Source） |
HEK293 |
|
表达序列（Sequence） |
Asp267-Ser375 |
|
Accession |
O14793 |
|
标签（Tag) |
No Tag |
|
分子量（Molecular Weight） |
The protein has a predicted MW of 12.40 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result. |
|
内毒素（Endotoxin） |
< 1 EU/μg by the LAL method. |
|
纯度（Purity） |
>95% as determined by Bis-Tris PAGE |
|
活性（Activity） |
ELISA Data 1: Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 0.13 μg/ml determined by ELISA . |
|
制剂（Formulation） |
Supplied as 0.22 μm filtered solution in 4 mM HCl. |
|
外观（Appearance） |
液体 |
储存条件
-85~-65℃保存，收到货之后有效期1年。避免反复冻融，打开小瓶前先离心。建议等分分装为一次实验使用量，分装后，-80℃冻存。
注意事项
1.为了您的安全和健康，请穿实验服并戴一次性手套操作。
2.本产品仅作科研用途。
产品数据
Cell Based Assay
Figure 1. Determined by its ability to inhibit the proliferation of MPC-11 cells.
The expected ED50 for this effect is <30 ng/ml
ELISA Data
Figure 2. Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 μg/ml (100 μl/well) on the plate.
Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 0.13 μg/ml determined
by ELISA.
SPR Data
Figure 3. Anti-GDF8 Antibody, hFc-Avi Tag captured on CM5 Chip via Protein A can bind
Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 49.45 pM as determined in
SPR assay.
Ver. CN20240919
