D-荧光素(D-Luciferin)是荧光素酶(Luciferase)的常用底物,普遍用于整个生物技术领域,特别是体内活体成像技术。其作用机制是在ATP和荧光素酶的作用下,荧光素(底物)能够被氧化发光。当荧光素过量时,产生的光量子数与荧光素酶的浓度呈正相关性。将携带荧光素酶编码基因(Luc)的质粒转染入细胞后,导入研究动物如大小鼠体内,之后注入荧光素,通过生物发光成像技术(BLI)来检测光强度变化,从而实时监测疾病发展状态或药物的治疗功效等。也可以利用ATP对此反应体系的影响,根据生物发光强度的变化来指示能量或生命体征。
中文别名(Chinese synonym) | D-荧光素钠盐; |
英文别名(English synonym) | (S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt; D-Luciferin firefly, sodium salt monohydrate; |
CAS号(CAS NO.) | 103404-75-7 |
分子式(Formula) | NaC11H7N2O3S2 · H2O |
分子量(Molecular weight) | 302.3 g/mol |
外观(Appearance) | 淡黄色粉末 |
溶解性(Solubility) | 溶于水(高达100 mg/ml) |
纯度(Purity)(HPLC) | ≥95.0% |
1、使用D-荧光素产品检测环状RNA在肿瘤生长中的作用机制。
用对照或 shcirc#1 或 #2 载体转导的 Huh7-Luc 细胞的原位肝肿瘤成像。
室温运输。-20℃干燥避光保存,有效期2年。
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