Q: 腔肠素溶液如何配制？

A: （1）腔肠素溶液配制方式来源文献，仅供参考，具体工作浓度建议参考文献做梯度摸索最佳浓度。 母液：称取 1mg 腔肠素粉末直接溶解于 197µl 酸化的甲醇溶液（ 100% 甲醇中含有  20µl/ml 3M  或者  6M HCl），配制成  12 mM （~5mg/ml,5×） 的腔肠素储存液。 工作液：如体外：10 的 5 次方的细胞数可用 10 μ M 。

Q:腔肠素的发光特性如何？

A: 体外：细胞孵育 10-15min 后，立即检测。 体内：可在注射后 10-15 min 内检测。

仅供参考，建议预实验建立荧光素酶动力学曲线，从而确定最高信号检测时间和信号平台期。

Q:是否能用于活体成像？

A: 一般用于体外，因为容易氧化，体内的话要注射和麻醉，时间久，体外检测快，不影响。体内的话推荐即用型。

Q:推荐仪器？多功能酶标仪能用吗？

A: 推荐仪器：具有生物化学发光检测模块。荧光素产生的光可以被光度计或闪烁计数器检测。常见的活体成像仪器：如 IVIS® Lumina 小动物活体成像系统，德国 Bruker 公司的 In-Vivo Xtreme 多模式小动物活体成像仪。多功能酶标仪：需要和仪器厂家确认是否具体生物化学发光检测模块。注：不能用荧光显微镜。

Q:说明书写到的配置储存液用的丙二醇是1,2-丙二醇吗？乙醇是无水乙醇吗？

A: 是的，丙二醇是1,2-丙二醇，乙醇是无水乙醇。

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