天然腔肠素(Coelenterazine native),又称腔肠素游离酸,是海肾荧光素酶(Rluc)和Gaussia荧光素酶(Gluc)等多种荧光素酶的作用底物,也是水母发光蛋白的辅助因子,应用非常广泛,可用于检测活细胞中钙离子浓度,基因报告分析,BRET(生物发光共振能量转移)研究,ELISA,HTS,以及组织或细胞中ROS水平的化学发光检测。
英文别名(English synonym) | Renilla luciferin;Coelenterazine free base |
CAS号(CAS NO.) | 55779-48-1 |
分子式(Formula) | C26H21N3O3 |
分子量(Molecular weight) | 423.46g/mol |
外观(Appearance) | 黄色固体 |
溶解性(Solubility) | 溶于甲醇和乙醇,不溶于DMSO |
纯度(Purity)(TLC) | >97% |
1、使用腔肠素产品探究可激活的报告系统 Rluc-SMN的体内成像效果
图1 体内成像结果展示
冰袋运输;粉末-20℃避光干燥保存,最好保存在惰性气体环境下,避免接触空气;长期保存于-70℃。有效期2年。
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