Specifications

Product name	Cat.No.	Size
Recombinant Human PDGF-CC Protein,His Tag	91606ES10	10 μg
	91606ES60	100 μg

 

Description

The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD.The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR-α and PDGFR-β, and are potent mitogens for a variety of cell types. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules' epithelial cell development.

 

Product information

Synonyms	PDGFCC; PDGF-CC
Source	E.coli
Molecular Weight	Approximately 27 kDa, a disulfide-linked homodimer of two 117 amino acid, C-terminal polyhistidine tagged proteins.
AA Sequence	Accession :NP_057289 Val235-Gly345,  with an N-terminal Met and 6-His tag;
Biological Activity	Measured in a cell proliferation assay using NR6R3T3 mouse fibroblast cells. The ED50 for this effect is 70-350 ng/mL.
Purity	> 97% by SDS-PAGE.
Endotoxin	<0.1EU per 1μg of the protein by the LAL method.
Formulation	Lyophilized from 0.2 µm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1 mg/mL. Further dilutions should be made in appropriately buffered solutions.

 

Storage

The product should be stored at -25~-15℃ for 1 year from date of receipt.

1 month, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Notes

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

                       

  Ver. CN20230630