Generation of an ELTD1 knockout human embryonic stem cell line by the iCRISPR/Cas9 system
Qian Luo, Cong Wei, Yan Long, Meng Zhang, Wei Shan, Honghu Li, Shuyang Cai, Yulin Xu, Pengxu Qian, He Huang
Journal：Stem Cell Research
IF：2.02
DOI：10.1016/j.scr.2021.102350
PMID：34087984
Published：2021-04-15
research field：肿瘤学分子生物学细胞生物学癌症治疗细胞应激反应生物化学
Abstract
Human ELTD1 (Epidermal growth factor, latrophilin and seven-transmembrane domain-containing 1), an orphan G-protein-coupled receptor (GPCR) belonging to the adhesion GPCR family, has been reported as a novel regulator of angiogenesis and a potential anti-cancer therapeutic target. However, little is known about the function of ELTD1, especially its undiscovered ligands. In this experiment, an ELTD1 homozygous knockout human embryonic stem cell line, FAHZUe001-A, was generated by the iCRISPR/Cas9 system to achieve a deeper understanding of ELTD1. The FAHZUe001-A was confirmed with normal karyotype, typical undifferentiated morphology, pluripotency and trilineage differentiation potential in vitro .
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