多孔Se@SiO2纳米球涂层导管通过ROS-NF-κB通路调控巨噬细胞极化促进前列腺尿道创面愈合

Abstract

Benign prostatic hyperplasia (BPH) patients experience complications after surgery. We studied oxidative stress scavenging by porous [email protected] 2 nanospheres in prostatic urethra wound healing after transurethral resection of the prostate (TURP). Beagle dogs were randomly distributed into two groups after establishing TURP models. Wound recovery and oxidative stress levels were evaluated. Re-epithelialization and the macrophage distribution at the wound site were assessed by histology. The mechanism by which porous [email protected] 2 nanospheres regulated macrophage polarization was investigated by qRT-PCR, western blotting , flow cytometry, immunofluorescence and dual luciferase reporter gene assays. Our results demonstrated that Porous [email protected] 2 nanosphere-coated catheters advance re-epithelization of the prostatic urethra, accelerating wound healing in beagle dogs after TURP, and improve the antioxidant capacity to inhibit oxidative stress and induced an M2 phenotype transition of macrophages at the wound. By restraining the function of reactive oxygen species (ROS), porous [email protected] 2 nanospheres downregulated Ikk, IκB and p65 phosphorylation to block the downstream NF-κB pathway in macrophages in vitro . Since activation of NF-κB signaling cascades drives macrophage polarization, porous [email protected] 2 nanospheres promoted macrophage phenotype conversion from M1 to M2. Our findings suggest that porous [email protected] 2 nanosphere-coated catheters promote postoperative wound recovery in the prostatic urethra by promoting macrophage polarization toward the M2 phenotype through suppression of the ROS-NF-κB pathway, attenuating the inflammatory response. Statement of Significance The inability to effectively control post-operative inflammatory responses after surgical treatment of benign prostatic hyperplasia (BPH) remains a challenge to researchers and surgeons, as it can lead to indirect cell death and ulti