中国军牧一号猪永生化卫星细胞系的建立与表征

Abstract

Simple SummarySkeletal muscle satellite cells are adult stem cells essential for muscle growth, repair, and meat production in livestock. However, primary satellite cells are difficult to maintain in laboratory culture because they stop dividing and spontaneously differentiate after only a few passages, limiting their use in research. The Junmu No.1 White pig is a Chinese commercial breed valued for its rapid growth, high lean meat yield, and strong adaptability, but no stable cell model is currently available for studying muscle development in this breed. In this study, we successfully established a long-term cultivable satellite cell line from Junmu No.1 piglets by introducing the SV40 large T antigen gene through lentiviral delivery. The resulting cell line maintained typical satellite cell features, normal chromosome numbers, and the ability to form muscle fibers in culture, while continuing to proliferate for more than 100 cell divisions. Importantly, its overall gene expression profile remained highly similar to that of primary cells. This new cell line provides a reliable and reproducible experimental tool for future research on muscle development, genetic regulation, and meat quality traits in Junmu No.1 pigs, and may also support broader applications in livestock biology and translational biomedical studies.Junmu No.1 is a commercially important Chinese pig breed, yet stable in vitro models for investigating its muscle development mechanisms and genetic regulation remain lacking; this study aimed to establish an immortalized porcine satellite cell line from Junmu No.1 pigs to address this gap. Primary porcine satellite cells (PSCs) were isolated from a 2-day-old Junmu No.1 piglet and immortalized via lentiviral transduction using the pHAGE-EF1α-eGFP-SV40LT-BleoR vector. The resulting cell line (imPSC-JM) was characterized for morphology, satellite cell marker expression, karyotype stability, myogenic differentiation capacity, and long-term proliferative po