分子别名（Synonyms）

CD279; PD1;PDCD1; PD-1

表达区间及表达系统（Source）

Human PD-1/PDCD1 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Leu25-Gln167.[Accession | Q15116-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 64-68 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized Human PD-1, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.17μg/ml determined by ELISA .Immobilized Human PD-1, mFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA.

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.