Synonyms

Growth Differentiation Factor 15,MIC-1

Uniprot No.

Q99988

Source

Human GDF-15 Protein is expressed from E. coli.It contains Ala197-Ile308

Molecular Weight

Predicted molecular mass of approximately 12 kDa in SDS-PAGE under reducing condition.

Biological Activity

Measured by its binding ability in a functional ELISA. When rHuGDF-15/MIC-1 is used at 0.5 µg/mL, the concentration of Recombinant Human Activin RIB/ALK-4 Fc Chimera that produces 50% of the optimal binding response is approximately 0.5-3 μg/mL.

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

<0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from 0.2 µm filtered concentrated solution in 4 mM HCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.5 mg/mL. Further dilutions should be made in appropriately buffered solutions.