产品信息
产品名称 |
产品编号 |
规格 |
YSFluorTM 488 Goat Anti-Rabbit lgG(H+L) |
33106ES60 |
100 μL |
产品简介
本品是由YSFluorTM 488标记的山羊抗兔IgG(H+L),使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子,也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白 发生交叉反应,但不会识别非免疫球蛋白类的血清蛋白。YSFluorTM 488,Amax(最大激发波长)为493 nm,Emax(最大发射波长)为519 nm,同Alex Fluor 488和FITC的光谱性质一样,通常可考虑相互替代。
本品适合做单标实验,广泛应用于多种免疫学实验如免疫细胞化学(ICC),流式细胞术(FC)以及免疫组化(IHC)等。要做多标(multiple-labeling)实验,建议使用与相近物种的血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。
产品应用
建议稀释浓度:1:50-1:400(For most application)
产品信息
抗体浓度(Antibody Concentration) |
100 μL(0.75 mg/mL) |
缓冲液(Buffer) |
0.005M 磷酸钠,0.125M 氯化钠,pH7.6 |
稳定剂(Stabilizer) |
7.5 mg/mL BSA(无IgG,蛋白酶), 50%甘油 |
防腐剂(Preservative) |
0.025%叠氮化钠 |
储存条件
-25 ~ -15℃分装保存,尽量避免反复冻融。
注意事项
Ver.CN20240314
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