A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

推荐稀释比 WB:1/3000; IP:1/200

2ug His fusion protein+ Primary antibody dilution at 1) 1:5000. 2) 1:10000

Immunofluorescence analysis of His Tag (2A2) in 293 cells transfected with a Histag protein using His Tag (2A2) antibody(red),and DAPI (blue).

Immunoprecipitation analysis of Flag Tag (7E1) in 1、untransfected 293 cell lysates2、transfected 293 cell lysates with Histag fusion protein3、IP (untransfected 293+antiHis mAb+ Protein G agarose)4、IP (transfected 293+ normal mouse IgG+Protein G agarose)5、IP (transfected 293+antiHis mAb+ Protein G agarose)

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.