A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

推荐稀释比 WB:1/1000-1/5000;IHC-P:1/1000-1/10000;ICC/IF:1/100;FC: 1/200-1/500;IP:1/20-1/50

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.