Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk2 is closely related to cdc2 (cdk1) which has proved useful as a marker of proliferation. Cdk1 and Cdk2 are a catalytic subunits of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle.

WB: 1/500-1/1000  IHC: 1/50-1/100  IP: 1/20  

Western

Western blot analysis of Cdk1/2 (Phospho-Thr14) in HepG2, U2OS, U251 lysates using Phospho-CDK1/2 (Thr14) antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cdk1/2 (Phospho-Thr14) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。