Myoglobin (MB) is an oxygen-binding protein that contains one polypeptide chain and one heme group. Reversible oxygen binding occurs by a linkage with the imidazole nitrogen of the 91st histidine residue in the myoglobin chain. Research studies indicate that the blockade of myoglobin in isolated cardiac myocytes mimics hypoxia when electrically stimulated for paced contractions. During fetal development, myoglobin is required to support cardiac function.

Western

Western blot analysis of Myoglobin in Human heart muscle lysates using Myoglobin antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using Myoglobin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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