产品简介
白细胞介素-3(Interleukin-3,IL-3)是一种造血生长因子,可促进巨核细胞、粒细胞-巨噬细胞、红系、嗜酸性粒细胞、嗜碱性粒细胞和肥大细胞谱系的定向祖细胞的存活、分化和增殖。IL-3由T细胞、肥大细胞和嗜酸性粒细胞产生,可增强血小板生成素、吞噬作用和抗体介导的细胞毒性。其激活单核细胞的能力表明IL-3可能具有额外的免疫调节作用。许多IL-3活性依赖于与其他细胞因子的共同刺激。
重组人IL-3是一种15.0kDa的重组蛋白,含有133个氨基酸残基。本品由大肠杆菌表达,以冻干粉形式提供,高活性、高纯度、低内毒素、无标签。
性能参数
别名(Synonyms) |
Interleukin-3; IL-3; Hematopoietic growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor |
表达系统(Source) |
E.coli. |
表达序列(Sequence) |
Ala20-Phe152. |
分子量(Molecular Weight) |
Approximately 15.08 kDa. |
Accession |
P08700 |
标签(Tag) |
No Tag |
生物活性(Biological Activity) |
The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7 × 106 IU/mg. |
纯度(Purity) |
> 95% as determined by SDS-PAGE. |
内毒素(Endotoxin) |
< 0.01EU/μg protein as determined by LAL method. |
无菌(Sterility) |
Negative. |
支原体检测(Mycoplasma) |
Negative. |
外源病毒(Vitro Virus) |
Negative. |
宿主残留蛋白(Host Cell Protein) |
Less than 0.5 ng/ug when tested by ELISA. |
宿主残留DNA(Host Cell DNA) |
Less than 20 ng/mg when tested by qPCR. |
制剂(Formulation) |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0. |
外观(Physical Appearance) |
Sterile filtered white lyophilized (freeze-dried) powder. |
储存条件
-25~-15℃保存,有效期3年。
复溶后, 2 ~8 ℃保存,30天有效期。复溶后, -85~-65℃保存,3个月有效期。
复溶方法
建议将Human IL-3在无菌PBS中复溶至0.1-1.0 mg/ml的浓度。尽可能避免反复冻融。
注意事项
1.为了您的安全和健康,请穿实验服并戴一次性手套操作。
2.本产品仅作科研用途。
产品数据
SDS- PAGE
Human IL-3 on SDS-PAGE under reduced condition.
The purity is greater than 95%.
Cell Based Assay
The ED50 as determined by a cell proliferation assay using human TF-1 cells
is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7 × 106 IU/mg.
Ver. CN20241022