Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

 

产品性质

别名	EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK
SwissProt ID	Q9NZJ5
基因ID	9451
免疫原	The antiserum was produced against synthesized peptide derived from human PEK/PERK around the phosphorylation site of Thr982. AA range:947-996.
缓冲液	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
分子量	Calculated MW: 58 kDa; Observed MW：58 kDa
克隆类型	Polyclonal Antibody
宿主	Rabbit
物种反应	Human, Mouse, Rat
推荐应用	WB, IHC, ICC/IF, ELISA
纯化方式	Affinity Purified

 

应用

稀释比 WB：1/500-1/1000；IHC: 1/50-1/100; IF: 1/50-1/200; ELISA: 1/10000

 

储存条件

-25~-15℃保存

 

注意事项

1. 避免反复冻融。

2. 本产品仅作科研用途。

2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

 

产品数据