Recombinant Human IL-18BP Protein,hFc Tag

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产品说明书

FAQ

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已发表文献

性能参数

表达区间及表达系统(Source

Recombinant Human IL-18BP Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Thr31-Gly194.[uniprot  | O95998-2]

分子量大小(Molecular Weight

The protein has a predicted MW of 44.4 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin

Less than 1EU per μg by the LAL method.

纯度(Purity

> 95% as determined by Tris-Bis PAGE and HPLC

活性(Activity

ELISA Data: Immobilized Human IL-18 at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 36.5ng/ml determined by ELISA   

SPR Data: Human IL-18BP, hFc Tag captured on Protein A chip, can bind Human IL-18 with an affinity constant of 0.04nM as determined in a SPR assay

制剂(Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.    

Ver. CN20230525

400-6111-883