性能参数
表达区间及表达系统(Source) |
Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-terminal. It contains Arg100-Ala219(NKG2A)&Ser34-Ile179 (CD94).[uniprot | P26715-1(NKG2A)&Q13241-1(CD94)] |
分子量大小(Molecular Weight) |
The protein has a predicted MW of 17.9 kDa (CD94)&41.1 kDa (NKG2A). Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by Tris-Bis PAGE and HPLC |
活性(Activity) |
ELISA Data: Immobilized Human NKG2A&CD94, hFc tag at 1μg/ml (100μl/Well) on Fc Antibody (5μg/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Ab., hFc Tag with the EC50 of 22.4ng/ml determined by ELISA. |
制剂(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法(Reconstitution) |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230525