This kit is consist of three different but reliable antibodies which are specific to the most popular three loading control targets: GAPDH, β-Actin and β-Tubulin, also with three individual package.
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression, and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer diseases.
推荐稀释比 WB:1/5000-1/10000;IHC:1/50-1/200;IF:1/100-1/200
Western blot analysis of beta Actin in HeLa, Human fetal kidney, NIH/3T3 and PC-12 lysates using beta Actin antibody.
Western blot analysis of beta Tubulin in MCF-7, COS1, Jurkat and HeLa lysates using beta Tubulin antibody.
Western blot analysis of GAPDH in Hela, Jurkat, mouse kidney, mouse spleen, RAW 264.7 and rat brain lysates using GAPDH antibody.
Immunohistochemistry analysis of paraffin-embedded Human heart, mouse testis, Human bladder cancer, mouse heart using beta Actin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunofluorescence analysis of beta Tubulin in NIH/3T3 using beta Tubulin antibody.
Immunohistochemistry analysis of paraffin-embedded rat colon, mouse testis, Human uterus, Human kidney using GAPDH antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Store at 4°C short term. Store at -20°C long term. Avoid freeze/thaw cycle.
