beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.

推荐稀释比 WB:1/500-1/1000;IHC:1/50-1/100;ELISA:1/10000

Western blot analysis of Actin in various lysates using Actin antibody.

Western blot analysis of Actin in HeLa lysates using Actin antibody.

Western blot analysis of Actin in various lysates using Actin antibody.

Western blot analysis of Actin in various lysates using Actin antibody.

Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using Actin antibody.High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody. was preabsorbed by immunogen peptide.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.