The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.

Western

Western blot analysis of CD99 in THP-1 (1), U937 (2), and MOLT4 (3) cell lysate using CD99 antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human ewing sarcoma tissues using CD99 antibody.

Immunocytochemistry

Immunocytochemistry analysis of CD99 in Hela cells using CD99 antibody (green),and DAPI(blue). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow

Flow Cytometry analysis of Jurkat cells using CD99 antibody (green) and negative control (red).

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