Abstract

A coccobacilli-shaped proteobacterium, designated strain SYSU XM001T, was isolated from an activated sludge sample collected from JiMei sewage treatment plant, Xiamen, PR China. Cells were Gram-stain-negative, catalase-positive and oxidase-negative. The 16S rRNA gene sequence of strain SYSU XM001Tshared less than 92 % sequence identities with members of the classAlphaproteobacteria, with highest sequence similarity toAquidulcibacter paucihalophilusTH1-2T(91.6 %; familyHyphomonadaceae, orderRhodobacterales). The strain exhibited growth at 25–37 °C, pH 7.0–9.0 and in the presence of up to 1 % (w/v) NaCl. Its chemotaxonomic features included ubiquinone-10 as the respiratory isoprenologue, iso-C16 : 0, 10-methyl C16 : 0TSBA and anteiso-C17 : 0as major cellular fatty acids and monoglycosyldiglyceride, glucuronopyranosyldiglyceride and two unidentified glycolipids as the main polar lipids. The DNA G+C content was determined to be 62.9 % (draft genome). Analyses of the phylogenetic data and differences in the chemotaxonomic and biochemical features from related genera in the familyHyphomonadaceaeindicated that strain SYSU XM001Tmerits representation of a novel species of a novel genus, for which the nameVitreimonas flagellatagen. nov., sp. nov. is proposed. The type strain ofVitreimonas flagellatais SYSU XM001T(=CGMCC 1.16661T=KCTC 62915T).