Abstract

Strain SYSU D8009Twas isolated from a desert sample collected from Saudi Arabia. The taxonomic position of the isolate was investigated by a polyphasic approach. The novel isolate was Gram-stain-negative, non-motile, aerobic and non-spore-forming. It was able to grow at 4–45 °C and pH 4.0–8.0, and exhibited NaCl tolerance of up to 1.5 % (w/v). Strain SYSU D8009Tshared the closest 16S rRNA gene sequence similarities with members of the familyAcetobacteraceae, with a value of less than 96.0 %. In the phylogenetic dendrograms, the strain clustered with the generaParacraurococcus,CraurococcusandCrenalkalicoccuswithin the familyAcetobacteraceaebut with a distinct lineage, thereby demonstrating that the strain should be classified within the familyAcetobacteraceae. The respiratory ubiquinone was found to be Q-10. The polar lipids of the strain comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and four unidentified aminolipids. The predominant cellular fatty acids were summed feature 8 (C18 : 1ω7cand/or C18 : 1ω6c) and C16 : 0. The genomic DNA G+C content of strain SYSU D8009Twas determined to be 71.6 mol%. Based on the results of the phylogenetic analyses and differences in the physiological and biochemical characteristics, strain SYSU D8009Tmerits representation of a novel species of a new genus within the familyAcetobacteraceae, for which the nameSiccirubricoccus desertigen. nov., sp. nov. is proposed. The type strain ofSiccirubricoccus desertisp. nov. is SYSU D8009T(=CGMCC 1.15936T=KCTC 62088T).