6×DNA上样缓冲液用于琼脂糖凝胶电泳或聚丙烯酰胺琼脂糖凝胶电泳前DNA 样本的处理。缓冲液组分经过优化,其中的染料溶液包括指示剂溴酚蓝和二甲苯青FF,电泳时可肉眼监控DNA 的迁移。甘油确保样本在点样孔底部聚集;EDTA结合二价金属离子并抑制金属离子依赖性核酸酶。
6×DNA上样缓冲液包括的各组分:30 mM EDTA、36%(v/v) Glycerol、0.05%(w/v) Xylene Cyanol FF、0.05%(w/v) Bromophenol Blue。
- 高性价比
室温运输。4℃可保存12个月,-20℃可长期保存。
[1] Qin W, Chen L, Wang Z, et al. Bioinspired DNA Nanointerface with Anisotropic Aptamers for Accurate Capture of Circulating Tumor Cells. Adv Sci (Weinh). 2020;7(19):2000647. Published 2020 Aug 9. doi:10.1002/advs.202000647(IF:15.840)
[2] Dai W, Zhang J, Meng X, et al. Catalytic hairpin assembly gel assay for multiple and sensitive microRNA detection. Theranostics. 2018;8(10):2646-2656. Published 2018 Apr 3. doi:10.7150/thno.24480(IF:8.537)
[20315ES]5×SDS-PAGE Protein Loading Buffer 5×SDS-PAGE蛋白上样缓冲液
[20316ES]5×Protein Loading Buffer (No Deturation Buffer) 5×非变性蛋白上样缓冲液
[20317ES]5×Protein Loading Buffer (No Deturation&Reducing Buffer) 5×非变性非还原蛋白上样缓冲液
[20319ES]10×Tris-Glycine-SDS电泳缓冲液 10×Tris-Glycine-SDS Running Buffer
[20320ES]4×Tris-HCl-SDS Separating Gel Buffer,pH8.8 4×Tris-HCl-SDS分离胶缓冲液,pH8.8
[20321ES]4×Tris-HCl Separating Gel Buffer, pH8.8 4×Tris-HCl分离胶缓冲液, pH8.8
[20322ES]4×Tris-HCl-SDS Stacking Gel Buffer,pH 6.8 4×Tris-HCl-SDS浓缩胶缓冲液,pH 6.8
[20323ES]4×Tris-HCl Stacking Gel Buffer, pH6.8 4×Tris-HCl浓缩胶缓冲液, pH6.8
[20329ES]Universal Electrophoresis and Transfer Buffer (for Western Blot) Western Blot电泳电转通用缓冲液