鬼笔环肽(Phalloidin)是一种来源于毒蕈类鬼笔鹅膏(Amanita phalloides)的环状七肽毒素,以高亲和力(Kd= 20 nM)选择性结合于丝状肌动蛋白F-actin,而不会与单体肌动蛋白G-actin结合,通常用来标记组织切片、细胞培养物或无细胞体系中的F-actin,从而对F-actin进行定性和定量分析。另外,鬼笔环肽衍生物也以相近的亲和力结合于大小纤维,无论是动植物来源的肌肉细胞或非肌肉细胞,按照每一个肌动蛋白亚基约与一个鬼笔环肽分子的计量比结合。且非特异性结合几乎可忽略,染色区域和非染色区域辨识度非常明显。因此,鬼笔环肽衍生物特别适合替代肌动蛋白(Actin)抗体进行相关研究。另外鬼笔环肽衍生物很小,直径约12-15Å,分子量<2000 Daltons,未标记肌动蛋白(Actin)的许多生理特性都得以维持,比如,同肌动蛋白结合蛋白如肌球蛋白,原肌球蛋白,DNase I等仍能发生反应;鬼笔环肽标记的纤维丝仍可穿透固相肌球蛋白基质;以及甘油抽提的肌纤维标记后仍可收缩等。
鬼笔环肽(Phalloidin)的结合阻止丝状肌动蛋白(微丝)的解离,稳定微丝结构,从而破坏微丝的聚合-去聚合的动态平衡。此特性使得肌动蛋白聚合发生的临界浓度(CC)降至<1 µg/mL,因此,可用作一种聚合促进剂。此外,鬼笔环肽还可抑制F-actin的ATP水解活性。
本品为iFluorTM 555标记的鬼笔环肽,可发出橘红色的荧光,具有高亮度和光稳定性,且染色反应特异性强,对比性高,具有比Actin抗体更好的染色效果,适合用作F-actin的定性和定量检测。iFluorTM 555 鬼笔环肽染色与用于细胞分析的其他荧光染色完全兼容,包括荧光蛋白、Qdot® 纳米晶体和其他iFluorTM偶联物(包含iFluorTM偶联二抗)。另外,经本品结合后的F-actin仍能维持actin自身具有的许多生物学特性。且本品的结合没有物种差异性,适用性广泛。本品以1 mg/mL浓度提供。
分子量(Molecular Weight) | ~1300 |
最大激发/发射波长(Ex/Em) | 556/574 nm |
溶解性(Solubility) | 溶于DMSO |
结构式(Structure) | ![]() |
冰袋运输。收到货后-20℃避光干燥保存,1年有效。
[1] Wang D, Zhao C, Xu F, et al. Cisplatin-resistant NSCLC cells induced by hypoxia transmit resistance to sensitive cells through exosomal PKM2. Theranostics. 2021;11(6):2860-2875. Published 2021 Jan 1. doi:10.7150/thno.51797(IF:11.556)
[2] Guo J, Li Y, Gao Z, et al. 3D printed controllable microporous scaffolds support embryonic development in vitro [published online ahead of print, 2022 Jun 14]. J Cell Physiol. 2022;10.1002/jcp.30810. doi:10.1002/jcp.30810(IF:6.384)
[3] Yang Y, Chen HY, Hao H, Wang KJ. The Anticancer Activity Conferred by the Mud Crab Antimicrobial Peptide Scyreprocin through Apoptosis and Membrane Disruption. Int J Mol Sci. 2022;23(10):5500. Published 2022 May 14. doi:10.3390/ijms23105500(IF:5.924)
[4] Ji Q, Ma J, Wang S, Liu Q. Systematic identification of a panel of strong promoter regions from Listeria monocytogenes for fine-tuning gene expression. Microb Cell Fact. 2021;20(1):132. Published 2021 Jul 12. doi:10.1186/s12934-021-01628-w(IF:5.328)
[5] Hou Y, Wu Z, Zhang Y, et al. Functional Analysis of Hydrolethalus Syndrome Protein HYLS1 in Ciliogenesis and Spermatogenesis in Drosophila. Front Cell Dev Biol. 2020;8:301. Published 2020 May 21. doi:10.3389/fcell.2020.00301(IF:5.186)
[6] Xiong X, Yang X, Dai H, et al. Extracellular matrix derived from human urine-derived stem cells enhances the expansion, adhesion, spreading, and differentiation of human periodontal ligament stem cells. Stem Cell Res Ther. 2019;10(1):396. Published 2019 Dec 18. doi:10.1186/s13287-019-1483-7(IF:4.627)
[7] Wang X, Huang S, Zheng C, Ge W, Wu C, Tse YC. RSU-1 Maintains Integrity of Caenorhabditis elegans Vulval Muscles by Regulating α-Actinin. G3 (Bethesda). 2020;10(7):2507-2517. Published 2020 Jul 7. doi:10.1534/g3.120.401185(IF:2.781)
[8] Sheng K, Li Y, Wang Z, Hang K, Ye Z. p-Coumaric acid suppresses reactive oxygen species-induced senescence in nucleus pulposus cells. Exp Ther Med. 2022;23(2):183. doi:10.3892/etm.2021.11106(IF:2.447)
[9] Yang Z, Gao X, Zhou M, et al. Effect of metformin on human periodontal ligament stem cells cultured with polydopamine-templated hydroxyapatite [published correction appears in Eur J Oral Sci. 2021 Aug;129(4):e12816]. Eur J Oral Sci. 2019;127(3):210-221. doi:10.1111/eos.12616(IF:1.810)