产品说明书
FAQ
COA
已发表文献
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
产品性质
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别名（Synonyms） |
HLA0201, MHC I, gp100, Glycoprotein 100, ME20-M, P100 |
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Uniprot No. |
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表达区间及表达系统（Source） |
Recombinant Human GP100(HLA-A*02:01) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and YLEPGPVTA peptide. |
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分子量(Molecular Weight) |
Approximately 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result. |
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外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
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活性（Activity） |
ELISA Data: Immobilized Human GP100 (HLA-A*02:01) Complex, His Tag at 2 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-GP100 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 32.9 ng/mL determined by ELISA. |
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内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
Tris-Bis PAGE
HB220706
