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已发表文献
该基因编码一种有效的细胞周期蛋白依赖激酶抑制剂。编码的蛋白结合并抑制细胞周期蛋白依赖的激酶2或-细胞周期蛋白依赖的激酶4复合物的活性，因此在G1时作为细胞周期进程的调节剂。该基因的表达受到肿瘤抑制蛋白p53的严格控制，该蛋白通过该蛋白介导p53依赖的细胞周期G1期阻滞，以应对各种应激刺激。该蛋白可与增殖的细胞核抗原(DNA聚合酶辅助因子)相互作用，在S期DNA复制和DNA损伤修复中发挥调节作用。据报道，该蛋白被casp3样caspases特异性切割，从而导致细胞周期蛋白依赖的激酶2的显著激活，并可能在caspase激活后细胞凋亡的执行中起作用。缺乏这种基因的老鼠具有再生受损或缺失组织的能力。该基因已发现多个交替剪接变异。
产品性质
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别名 |
CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21 |
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SwissProt ID |
P38936 |
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基因ID |
1026 |
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免疫原 |
The antiserum was produced against synthesized peptide derived from human p21 Cip1. AArange:111-160 |
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缓冲液 |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3 |
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分子量 |
Observed MW (kDa):26 |
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克隆类型 |
Polyclonal Antibody |
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亚型 |
IgG |
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宿主 |
Rabbit |
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物种反应 |
Human,Mouse,Rat |
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纯化方式 |
Affinity Purified |
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细胞定位 |
细胞质，细胞核 |
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研究领域 |
表观遗传学，细胞生物学与发育生物学，癌症，内分泌代谢 |
应用
稀释比IF: 1/50-200 WB: 1/500-1/2000 ELISA: 1/20000
 |
Western Blot analysis of various cells using p21 Polyclonal Antibody |
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Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,p21 Polyclonal Antibody was diluted at1:2 (4°C,overnight). 2, Sodium citrate pH 6. was used for antibodyretrieval (>98°C,2min). 3,Secondary antibody was diluted at 1:2 (room tempeRature, 3min).Negative control was used by secondary antibody only |
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Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,p21 Polyclonal Antibody was diluted at 1:200 (4°C,overnight) . 2, Sodium citrate pH 6.0 was used forantibody retrieval (>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,p21 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used forantibody retrieval (>98°C,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only. |
运输与保存方法
冰袋运输。-20℃保存，1年有效期。
注意事项
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3. 本产品仅作科研用途！
HB220607
