This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

推荐稀释比 WB:1/500-1/1000; IHC:1/50-1/100; IF:1/50-1/200

Western blot analysis of Collagen I alpha 1 in various cell lines lysates using COL1A1 antibody.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using COL1A1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunohistochemistry analysis of paraffin-embedded Human placenta using COL1A1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunohistochemistry analysis of paraffin-embedded rat ovary using COL1A1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence analysis of Collagen I alpha 1 in NIH/3T3 using Collagen I alpha 1 antibody ,and DAPI (blue).

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.